Become A Master in HPLC Technique - (Liquid Chromatography) (Udemy.com)

Dear Friend,

This course is the first and only dedicated HPLC course exclusively on Udemy.

High-Performance Liquid Chromatography (HPLC) is one of the most widely used analytical tools in testing laboratories. It allows for the separation, identification, purification, and quantification of drug substances as well as many organic compounds.

HPLC is an incredibly powerful technique with applications across multiple fields: analytical chemistry, pharmaceuticals, food and nutrition, polymers, agricultural sciences, environmental testing, and research in universities and industry.

For students who complete training in HPLC, there are many exciting career opportunities, including:

• R&D scientists

• QA managers and practicing chemists

• Researchers in chemistry, pharmacy, food and nutrition, polymer science, and agricultural sciences

• Life science researchers

• Professionals in governmental and regulatory bodies

• Environmental science specialists

The ability to operate HPLC is now considered a fundamental skill for chemists and technicians in laboratories. Recent surveys from industry and academia highlight a strong demand for HPLC training, especially for diploma graduates and working professionals who intend to join testing labs but lack hands-on training opportunities. This course was designed to fill that gap by providing a comprehensive and practical introduction to HPLC concepts, operation, and applications.

Student Reviews

★★★★★ “I’m taking this course to complete my PhD research in Analytical Chemistry. I prepared a new organic compound and needed a method for analysis. This will increase my publication opportunities, and my supervisor is pleased. HPLC is the best method for my work.” – Forhad

★★★★★ “Finally I found what I was looking for. This course covers all the basics in HPLC. I learned so much more than in my school. Very helpful and I’m glad I took this course.” – Jonlok

★★★★★ “Outstanding experience. This course helped me increase my confidence for future job applications in the pharmaceutical industry. Now I can apply with the knowledge and certificate from this course.” – Akef Abuinsair

★★★★★ “This course covers essential information about chromatography. The explanations are step-by-step and easy to follow. Excellent course!” – Reloex

★★★★★ “A very comprehensive and well-balanced HPLC course. The instructor has excellent experience in HPLC and explains everything clearly.” – Alex

What You’ll Learn

This course will guide you through everything you need to know about HPLC and how it works. You will learn about the components of this instrument, the importance of the stationary phase (the column), and the chemistry behind separations. The content is designed for learners of all levels, regardless of prior knowledge.

I am Dr. Mohammad Abualrub, PhD in Chemistry, with more than 8 years of experience in the pharmaceutical industry and over 4 years of experience teaching chemistry. I have been using HPLC daily in laboratory settings for more than 8 years, and I bring this expertise into the course.

Course Content

• Introduction to liquid chromatography

• How HPLC works

• HPLC analysis

• Components of HPLC

• Column chemistry – stationary phase

• System suitability

• Starting and setting up the HPLC system

• Determination of assay using HPLC

• Method development

You will also get the chance to see a real HPLC instrument inside an analytical chemistry lab. I will demonstrate how to install a column, prepare the mobile phase, and run the system step by step.

New! HPLC Practice Test (30 Questions)

To strengthen your learning, this course now includes a dedicated 30-question HPLC Practice Test.

• Covers all major topics: theory, components, column chemistry, assay, system suitability, and method development.

• Designed in multiple-choice format, just like professional exams.

• Helps you evaluate your understanding, identify weak areas, and build exam-style confidence.

• Estimated completion time: 40 minutes.

At the End of This Course

You will test your knowledge with quizzes and the final practice test covering essential questions such as:

• What does HPLC stand for?

• Typical HPLC column dimensions

• Pressure range in normal analytical separations

• Commonly used mobile phases in HPLC

• Effect of flow rate on separation

• Materials used in HPLC columns

• Most commonly used HPLC detectors

• Isocratic vs gradient operation

• How to control retention time drift

This course is risk-free with Udemy’s 30-day money-back guarantee. If you feel you have gained no value, you can request a refund with no questions asked. I stand fully behind this training and am confident it will give you the skills you need.

Let’s start success together. Click on “Take this course” at the top right of this page and begin your journey in mastering HPLC today.

Dr. Mohammad Abualrub
PhD in Chemistry
Expert in the Pharmaceutical Industry (+8 Years)
Expert in Teaching Chemistry (+4 Years)